DSS诱导斑马鱼溃疡性结肠炎模型的建立-技术前沿-资讯-生物在线

DSS诱导斑马鱼溃疡性结肠炎模型的建立

作者:翌圣生物科技(上海)股份有限公司 2024-07-12T00:00 (访问量:30774)

斑马鱼作为肠道损伤模型的应用已得到充分证实,葡聚糖硫酸钠(Dextran Sulfate Sodium Salt,DSS)诱导的斑马鱼IBD模型模拟了哺乳动物IBD表型的不同特征,包括肠道中性粒细胞炎症、过多粘液生成、肠淋巴管生成增加以及前炎性细胞因子上调。 

图片来源:包图网
 

实验动物

3dpf(days post fertilization) 斑马鱼

 

实验材料

DSS(Yeasen 60316ES,MW:36000~50000)

 

实验步骤

                                       1. 将斑马鱼胚胎培养在含甲基蓝的E3胚胎培养基中,28.5 ℃,培养至1 dpf;

                                       2. 1dpf 后使用不含亚甲基蓝的E3胚胎培养基,培养至3 dpf;

                                       3. 用E3培养基配制浓度为10% DSS的储存液;

                                       4. 用培养基稀释DSS至最大非致死剂量(DSS浓度参考:0.5%);

                                       5. 用0.5%的DSS处理斑马鱼,从3 dpf处理到6 dpf,观察指标[1]

 

结果分析

                                       造模成功指标:中性粒细胞数量增多、酸性粘液蛋白增多、炎性因子表达量上升。

                                       1.嗜中性粒细胞数量:中性粒细胞数量增多,并且向肠和上皮中移动

                                       2.酸性粘液蛋白:石蜡病理切片结合AB-PAS染色法验证,DSS浸泡后斑马鱼肠道中酸性粘液蛋白增多

                                       3.炎性因子表达量:DSS处理后斑马鱼肠道中炎性因子表达量上升[1,2]

 

客户案例

图1.Yeasen DSS(Cat#60316ES)斑马鱼造模实验结果(Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557)

 

DSS处理组相较于对照组,酸性粘蛋白染色和粘液分泌增加,斑马鱼外周血中的嗜中性粒细胞数量增多并且向肠和上皮中移动,炎性因子表达量显著上调,肠道出现炎症反应。

 

翌圣DSS产品优势

 翌圣提供高品质DSS(Cat.NO:60316ES,MW:36000~50000),具有以下优势:

1.高纯度(>98%),硫含量17-19%,游离硫<0.2%,大量数据文献支持,广泛应用于UC模型的构建;

2.多种属动物中均可造模:小鼠、大鼠、斑马鱼、猪、果蝇;

3.症状表现与人UC极度相似:可用于研究急、慢性结肠炎的发生发展机制,也可用于药物的药效研究;

4.简单易行,成模率高,重复性强;

5.联合氧化偶氮甲烷 (azoxymethane, AOM)用药,可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型,成功模拟IBD诱发CAC的过程。

                                           建模效率与进口产品一致,产品价格低至进口价格的1/3,大量现货库存。
 

产品订购

产品名称

货号

规格

价格(元)

促销价(元)

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES25

25 g

1255

955

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES60

100 g

3855

2655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES76

500 g

16855

9655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES80

1 kg

32855

18655

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产品已发表文章(部分)

 

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2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

 

2021

[1] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

 

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

 

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943

 

 

相关专题

1.不同分子量葡聚糖硫酸钠选购指南:高品质DSS葡聚糖硫酸钠盐

2.DSS结肠炎造模整体解决方案:Dextran Sulfate Sodium Salt(DSS) 溃疡性结肠炎模型的建立 (yeasen.com) 



参考文献:[1] Oehlers SH, et al. Dis Model Mech. 2012 Jul;5(4):457-67.[2] Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557.

 

 

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